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Journal of Veterinary Diagnostic Investigation, Vol 13, Issue 1, 13-16
Copyright © 2001 by American Association of Veterinary Laboratory Diagnosticians

Articles

Immunohistochemical detection of avian pneumovirus in formalin-fixed tissues

FE Jirjis, SL Noll, DA Halvorson, KV Nagaraja, and DP Shaw

Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, Saint Paul 55108, USA.

An immunohistochemical staining technique (IHC) was developed to detect avian pneumovirus (APV) antigen in formalin-fixed, paraffin-embedded tissue sections using streptavidin-biotin immunoperoxidase staining. Samples of nasal turbinates and infraorbital sinuses were collected from 4-week-old poults experimentally inoculated with APV and from older turkeys infected during naturally occurring outbreaks of avian pneumovirus. Tissue was fixed in 10% buffered neutral formalin, embedded in paraffin, sectioned and stained. Inflammatory changes were observed microscopically in the mucosa and submucosa of the nasal turbinates and infraorbital sinuses of both experimentally inoculated poults and naturally infected birds. Viral antigen was detected by IHC in the ciliated epithelial cells of nasal turbinates and infraorbital sinuses.


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R. S. Bennett, R. LaRue, D. Shaw, Q. Yu, K. V. Nagaraja, D. A. Halvorson, and M. K. Njenga
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