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Journal of Veterinary Diagnostic Investigation, Vol 13, Issue 1, 17-21
Copyright © 2001 by American Association of Veterinary Laboratory Diagnosticians

Clinical Trial

An indirect enzyme-linked immunosorbent assay for diagnosis of American canine hepatozoonosis

JS Mathew, JT Saliki, SA Ewing, TW Lehenbauer, RJ Panciera, Malayer JR, CA Cummings, and AA Kocan

College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.

American canine hepatozoonosis (ACH), caused by Hepatozoon americanum, is an emerging tick-borne disease of dogs. An indirect enzyme-linked immunosorbent assay (ELISA) that should facilitate diagnosis of infection and study of the epidemiology of ACH has been developed using H. americanum sporozoites as antigen. Efficacy of the new test as a diagnostic tool was compared with that of skeletal muscle biopsy, the current gold standard for confirming H. americanum infection. Results show that the test is sensitive (93%) and specific (96%) and that it is as reliable as histopathologic examination of skeletal muscle for detecting infection. The ELISA would be suitable as a routine laboratory test for diagnosis of ACH.


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 Clin. Microbiol. Rev.Home page
S. A. Ewing and R. J. Panciera
American Canine Hepatozoonosis
Clin. Microbiol. Rev., October 1, 2003; 16(4): 688 - 697.
[Abstract] [Full Text] [PDF]

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 Vet PatholHome page
R. J. Panciera, J. S. Mathew, C. A. Cummings, J. C. Duffy, S. A. Ewing, and A. A. Kocan
Comparison of Tissue Stages of Hepatozoon americanum in the Dog Using Immunohistochemical and Routine Histologic Methods
Vet. Pathol., July 1, 2001; 38(4): 422 - 426.
[Abstract] [Full Text] [PDF]


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