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Journal of Veterinary Diagnostic Investigation, Vol 13, Issue 5, 389-393
Copyright © 2001 by American Association of Veterinary Laboratory Diagnosticians

Articles

The use of a neutralizing monoclonal antibody to detect infections of equine herpesvirus type 2 (EHV-2)

A Nordengrahn, B Klingeborn, A Lindholm, and M Merza

SVANOVA Biotech, National Veterinary Institute (SVA), Uppsala Science Park, Sweden.

A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to equine herpesvirus 2 in serum samples of horses. By measuring the binding to a single epitope, this blocking ELISA gives a good picture of the antibody status in the animal. The test is based on a monoclonal antibody with neutralizing activity and had a sensitivity of 94% and a specificity of 100%. Antibodies due to newly acquired infection in foals were successfully detected with this blocking ELISA.




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Copyright © 2001 by the American Association of Veterinary Laboratory Diagnosticians, Inc.