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Journal of Veterinary Diagnostic Investigation, Vol 14, Issue 1, 40-46
Copyright © 2002 by American Association of Veterinary Laboratory Diagnosticians

Articles

Polymerase chain reaction and DNA sequencing for detection of ovine herpesvirus 2 in American bison (Bison bison)

EA Sausker and NW Dyer

Genaissance Pharmaceuticals, 5 Science Park, New Haven, CT 06511, USA.

Serum samples were collected at slaughter from 226 24-30-month-old ranch-raised, clinically normal American bison (Bison bison) bulls from North Dakota, Minnesota, Kansas, and Manitoba to assess the presence of antibodies to ovine herpesvirus 2 (OHV-2). Antibodies to OHV-2 were detected by competitive inhibition enzyme-linked immunosorbent assay in 10 of 226 (4.40%) samples. Polymerase chain reaction (PCR) analysis of sera positive for OHV-2 DNA demonstrated a 238 kilobase fragment. The nucleotide sequence of the PCR-positive samples in comparison to the reported OHV-2 nucleotide sequence resulted in a homology range of 82.8-95.4%.




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Copyright © 2002 by the American Association of Veterinary Laboratory Diagnosticians, Inc.