JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frazier, K.
Right arrow Articles by Hines, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frazier, K.
Right arrow Articles by Hines, M., 2nd
Journal of Veterinary Diagnostic Investigation, Vol 14, Issue 6, 457-462
Copyright © 2002 by American Association of Veterinary Laboratory Diagnosticians

Articles

Seroprevalence and comparison of isolates of endometriotropic bovine herpesvirus-4

KS Frazier, CA Baldwin, M Pence, J West, J Bernard, A Liggett, D Miller, and ME Hines 2nd

College of Veterinary Medicine, Tifton Veterinary Diagnostic and Investigational Laboratory, University of Georgia, 31973, USA.

Sixty-eight cases of suppurative, ulcerative endometritis associated with Bovine Herpesvirus-4 (BHV-4) in postparturient dairy cows (62 Holsteins and 6 Jerseys, mean age 4.2 years) were confirmed by a combination of histopathology, fluorescent antibody assays, electron microscopic evaluation of uterus, and polymerase chain reaction (PCR). All cases occurred in the 3- to 28-day postpartum period, and histologic lesions among various cows were consistent when compared with postpartum interval. The endometrial lining epithelium was necrotic and ulcerated from 3 to 7 days postpartum, with only mild inflammation in the lamina propria and submucosa. From 1 to 4 weeks postpartum, the ulcers were confluent to diffuse. Epithelium was replaced by fibrinonecrotic, suppurative mats, resulting in severe bacterial pyometra by day 24. Seroprevalence to BHV-4 in one dairy with a history of 18 mortality cases was 36% (107 of 296). In a random sample of 8 cows from this herd, none had serologic titers in blood sampled 2 weeks prepartum, but 3 of 8 seroconverted with significant titers of 1:8 to 1:16 at 2 weeks postpartum. By 10 weeks postpartum, all 8 cows returned to negative serologic status. Two of 6 cats from the premises also had positive titers. Random serum samples taken from 480 dairy cattle at sale barns indicated 76 (16%) were positive by serum neutralization. Clinical signs, postparturient timing, and histologic lesions were very similar to those previously reported in Belgium with BHV-4. But sequence analysis of PCR products of the glycoprotein B region of 4 separate field isolates of endometriotropic BHV-4 suggests these field isolates were more closely related to the North American nonvirulent strain DN-599 than to the endometriotropic European strain V.


This article has been cited by other articles:


Home page
 ReproductionHome page
G. Donofrio, L. Ravanetti, S. Cavirani, S. Herath, A. Capocefalo, and I. M. Sheldon
Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation: a new insight into bacterial and viral interaction for uterine disease
Reproduction, September 1, 2008; 136(3): 361 - 366.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
G. Donofrio, S. Herath, C. Sartori, S. Cavirani, C. F. Flammini, and I. M. Sheldon
Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function
Reproduction, July 1, 2007; 134(1): 183 - 197.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
G. Donofrio, S. Cavirani, V. van Santen, and C. F. Flammini
Potential Secondary Pathogenic Role for Bovine Herpesvirus 4
J. Clin. Microbiol., July 1, 2005; 43(7): 3421 - 3426.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association of Veterinary Laboratory Diagnosticians, Inc.