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Journal of Veterinary Diagnostic Investigation, Vol 14, Issue 6, 523-525
Copyright © 2002 by American Association of Veterinary Laboratory Diagnosticians

Articles

Isolation of bovine viral diarrhea virus from an alpaca

SM Goyal, M Bouljihad, S Haugerud, and JF Ridpath

Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.

A frozen, stillborn alpaca (Lama pacos) was submitted to the Minnesota Veterinary Diagnostic Laboratory for diagnostic purposes. No gross or histopathologic changes of any significance were seen. A pool of lung, liver, and brain tissues was positive for bovine viral diarrhea virus (BVDV) by reverse transcription-polymerase chain reaction. On inoculation in cell cultures, a noncytopathic BVDV (type 1b) was isolated. No evidence of BVDV was seen on immunohistochemical examination of tissues. This indicates the importance of using multiple tests for arriving at a diagnosis and appears to be the first report of BVDV isolation from alpaca.


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