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Journal of Veterinary Diagnostic Investigation, Vol 17, Issue 4, 350-354
Copyright © 2005 by American Association of Veterinary Laboratory Diagnosticians

Articles

Performance of a Johne's disease enzyme-linked immunosorbent assay adapted for milk samples from goats

M Salgado, EJ Manning, and MT Collins

University of Wisconsin, School of Veterinary Medicine, 2015 Linden Drive, Madison, WI 53706-1102, USA.

Antibody detection-based tests for paratuberculosis offer speed and economy, 2 diagnostic test attributes important to animal industries with narrow profit margins. Application of such tests to individual milk samples instead of serum samples can further improve testing efficiency and decrease testing cost. Accuracy of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay (ELISA) adapted for use on goat serum and milk samples was determined. Fecal, blood, and milk samples were collected from 159 goats belonging to 2 Wisconsin goat herds with a prior history of paratuberculosis and 1 herd of 50 goats from a paratuberculosis-free Wisconsin herd. Fecal samples were cultured using the modified BACTEC 12B media. Sera were tested according to the manufacturer's instructions for bovine samples. Milk samples were centrifuged and mixed with the ELISA kit's Mycobacterium phlei-containing diluent at a ratio of 1:2. Using fecal culture as the "gold standard," the sensitivity of the ELISA on goat serum was 64% and the sensitivity of the ELISA on goat milk was 48%. The milk ELISA had higher agreement with fecal culture results (kappa = 0.525) than the serum ELISA (kappa = 0.425). ELISA specificity was 100% on both serum and milk. Regression analysis also showed good correlation between serum and milk S/P values (r2 = 0.67). Although less sensitive, the ELISA on goat milk samples appears to offer a useful, low-cost alternative for detection of goats with paratuberculosis that have progressed to the stage of shedding M. paratuberculosis in their feces.


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E. J. B. Manning,, H. F. Cushing, S. Hietala, and C. Wolf
Impact of Corynebacterium pseudotuberculosis infection on serologic surveillance for Johne's disease in goats
J Vet Diagn Invest, March 1, 2007; 19(2): 187 - 190.
[Abstract] [Full Text] [PDF]

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M. Salgado, J. Kruze, and M. T. Collins
Diagnosis of paratuberculosis by fecal culture and ELISA on milk and serum samples in two types of Chilean dairy goat herds
J Vet Diagn Invest, January 1, 2007; 19(1): 99 - 102.
[Abstract] [Full Text] [PDF]

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J. Kruze, M. Salgado, E. Paredes, A. Mella, and M. T. Collins
Goat Paratuberculosis in Chile: First Isolation and Confirmation of Mycobacterium Avium Subspecies Paratuberculosis Infection in a Dairy Goat
J Vet Diagn Invest, September 1, 2006; 18(5): 476 - 479.
[Abstract] [Full Text] [PDF]


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