HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Grego, E. Articles by Rosati, S. Search for Related Content PubMed PubMed Citation Articles by Grego, E. Articles by Rosati, S.

Development and application of an enzyme-linked immunosorbent assay for detection of bovine viral diarrhea antibody based on E^rns glycoprotein expressed in a baculovirus system

Correspondence: ¹ Corresponding Author: Sergio Rosati, Dipartimento di Produzioni Animali, Epidemiolgia ed Ecologia, Facoltà di Medicina Veterinaria, Via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy, e-mail: sergio.rosati{at}unito.it

The BVDV envelope glycoprotein E^rns/gp48 and the C terminal 79 amino acids of the capsid protein coding region were expressed in a baculovirus system and antigenically characterized. Western blot assay was used to detect recombinant E^rns (r-E^rns) in infected insect cells using specific monoclonal antibodies. The r-E^rns was then used in an indirect ELISA to detect BVDV specific antibodies in a panel of 540 well-characterized sera. Results of the r-E^rns ELISA were compared to those obtained with a commercially available competitive ELISA targeting anti-NS2/3 antibodies. A good correlation was observed between the 2 ELISA (kappa = 0.916, 95% C.I.: 0.876, 0.956). Using the commercial NS2/3 ELISA as the reference test, the relative sensitivity of r-E^rns ELISA was 97.5% (95% C.I.: 94.3%, 99.1%) and the relative specificity was 93.9% (95% C.I.: 89.4%, 96.9%), while relative specificity was 100% (95% C.I.: 97%, 100%) using true negative sera (derived from a negative herd). All but 1 antigen positive animals (n = 36) tested negative in the r-E^rns ELISA; among them all 22 confirmed PI animals were negative by r-E^rns ELISA. The ability of r-E^rns ELISA to identify cattle immunized with inactivated vaccine was also demonstrated in a small group of cattle, compared to an NS2/3 antibody ELISA. Results suggest that r-E^rns ELISA represents an alternative test for antibody generated by natural infection or BVDV vaccination.

Key Words: Baculovirus • bovine viral diarrhea virus • ELISA • E^rns glycoprotein