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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 1, 78-83
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communications

Genetic characterization of canine rotavirus isolated from a puppy in korea and experimental reproduction of disease

Bo Kyu Kang, Dae Sub Song, Kwon Il Jung, Chul Seung Lee, Sung Jun Park, Jin Sik Oh, Dong Jun An, Jeong Sun Yang, Hyoung Joon Moon, Sang Sun Lee, Young Dhuk Yoon and Bong Kyun Park1

Correspondence: 1Corresponding Author: Dr. B.K Park, Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea, e-mail: parkx026{at}snu.ac.kr

Canine rotavirus was isolated from feces of a Korean Jindo dog with mild diarrhea, and the isolate was genetically characterized. Rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. The virus isolate (GC/KS05) was identified as subtype G3P[3] using reverse transcription polymerase chain reaction (RT-PCR). The strain displayed 98% and 90% identity with the VP7 genes of a canine rotavirus isolate (RV52/96) from Italy and the simian rotavirus strain (RRV) respectively. However, the GC/KS05 isolate exhibited only 83% and 82% identity, respectively, with the G3 serotype canine strains, RV198/95 and K9. Phylogenetic analysis of the VP7 and VP4 genes of GC/KS05 strain led to the classification of VP7 in a different cluster than other canine rotavirus VP7 genes, and VP4 within the cluster of canine rotavirus VP4 genes. The Korean isolate was thus more closely related to the RV52/96 isolate than the other isolates for which sequence data is available. Detailed analysis of the VP7 region revealed 6 amino acid variations between the new isolate and RV52/96. After 5 passages in cell culture, the GC/KS05 strain remained pathogenic for young pups, in which inoculation resulted in diarrhea and virus shedding in the feces.

Key Words: Canine rotavirus • genotype G3P[3]






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