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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 1, 84-87
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communications

Comparative serological study of Leptospira serovar hardjo genotypes for use in the microscopic agglutination test

Morgan L. Hotka1, Mark A. Wilson, Tammy M. Anderson, Corrie L. Tichenor and David A. Miller

Correspondence: 1Corresponding Author: Morgan Hotka, 1800 Dayton Avenue, Ames, IA 50010, e-mail: Morgan.L.Hotka{at}aphis.usda.gov

A comparative serological study was conducted using the Leptospira microscopic agglutination test (MAT). Genotypes hardjoprajitno (HP), hardjo-bovis A (HA), and hardjo-bovis B (HB) were compared to determine which best detects hardjo antibody in cattle serum. A total of 2,431 cattle sera were tested. Sera were collected from 4 geographic regions of the United States. Samples were obtained without knowledge of breed, age, vaccination history, or herd health status. Of the sera collected, 60.7% (1,475) were negative at the 1 : 100 dilution for all three genotypes. Serological reactivity at the 1 : 100 dilution was identified in 956 (39.3%) of the sera tested. Considering the 956 positive sera, 941 (98.4%) reacted to HP, whereas the remaining 15 sera (1.6%) reacted to only HA and/or HB. A total of 394/941 (41.9%) HP positive sera failed to react to HA or HB. The results of this study support the conclusion that HP antigen was most sensitive in detection of hardjo antibody.

Key Words: Cattle • hardjo • hardjo-bovis • hardjoprajitno • Leptospira • MAT • serology




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S. Rajeev, R. D. Berghaus, M. W. Overton, M. E. Pence, and C. A. Baldwin
Comparison of fluorescent antibody and microscopic agglutination testing for Leptospira in pregnant and nonpregnant cows
J Vet Diagn Invest, January 1, 2010; 22(1): 51 - 54.
[Abstract] [Full Text] [PDF]


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