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Comparative serological study of Leptospira serovar hardjo genotypes for use in the microscopic agglutination test

Correspondence: ¹Corresponding Author: Morgan Hotka, 1800 Dayton Avenue, Ames, IA 50010, e-mail: Morgan.L.Hotka{at}aphis.usda.gov

A comparative serological study was conducted using the Leptospira microscopic agglutination test (MAT). Genotypes hardjoprajitno (HP), hardjo-bovis A (HA), and hardjo-bovis B (HB) were compared to determine which best detects hardjo antibody in cattle serum. A total of 2,431 cattle sera were tested. Sera were collected from 4 geographic regions of the United States. Samples were obtained without knowledge of breed, age, vaccination history, or herd health status. Of the sera collected, 60.7% (1,475) were negative at the 1 : 100 dilution for all three genotypes. Serological reactivity at the 1 : 100 dilution was identified in 956 (39.3%) of the sera tested. Considering the 956 positive sera, 941 (98.4%) reacted to HP, whereas the remaining 15 sera (1.6%) reacted to only HA and/or HB. A total of 394/941 (41.9%) HP positive sera failed to react to HA or HB. The results of this study support the conclusion that HP antigen was most sensitive in detection of hardjo antibody.

Key Words: Cattle • hardjo • hardjo-bovis • hardjoprajitno • Leptospira • MAT • serology