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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 3, 244-249
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

An Indirect Enzyme-linked Immunosorbent Assay for Detection of Antibodies to Actinobacillus Pleuropneumoniae Serovar 7 in Pig Serum

Joan Klausen1, Lars Ekeroth, Jan Grøndahl-Hansen and Lars Ole Andresen

Correspondence: 1Corresponding Author: Dr. Joan Klausen, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. , e-mail: jk{at}vet.dtu.dk

Lipopolysaccharide (LPS) antigen was purified from Actinobacillus pleuropneumoniae serovar 7 by phenol–water extraction and fractionated on a, S-100 Sephacryl column. High molecular weight fractions of LPS purified from the S-100 column were pooled and used as antigen in an indirect serovar 7 ELISA. The ELISA was evaluated with sera from pigs experimentally infected with 11 different A. pleuropneumoniae serovars of biotype 1. Estimation of sensitivity and specificity of the A. pleuropneumoniae serovar 7 ELISA was performed using pig sera from herds naturally infected with A. pleuropneumoniae serovar 7 as well as sera from herds free of infection with A. pleuropneumoniae serovar 7. When compared to the complement fixation test (CFT) as a reference test, the ELISA showed much higher sensitivity and statistically equivalent specificity.

Key Words: Actinobacillus pleuropneumoniae • ELISA • Lipopolysaccharide • serological assay




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