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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 3, 298-300
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communication

Failure to Detect Bovine Viral Diarrhea Virus in Necropsied Farm-raised White-tailed Deer (Odocoileus Virginianus) in Pennsylvania

Jason W. Brooks1, Douglas W. Key, Arthur L. Hattel, Ernest P. Hovingh, Ryan Peterson, Daniel P. Shaw and Jenny S. Fisher

Correspondence: 1Corresponding Author: Jason W Brooks, Animal Diagnostic Laboratory, The Pennsylvania State University, Orchard Road, University Park, PA 16802, e-mail: jwb21{at}psu.edu

Between January 1 and December 31, 2005 gross and histologic examinations were performed on carcasses of 61 farm-raised white-tailed deer originating from Pennsylvania. Single-tube real-time reverse transcription polymerase chain reaction (real-time RT-PCR) for the detection of bovine viral diarrhea virus type 1 (BVDV-1) and type 2 (BVDV-2) was performed on each animal. Virus isolation was performed on tissue samples from 25 of 61 animals. Immunohistochemical (IHC) staining of ear-notch skin to identify BVDV antigen was performed on each animal. All tissues samples tested negative for both BVDV-1 and BVDV-2 by real-time RT-PCR, virus isolation, and IHC. Gross or histopathologic lesions suggestive of BVDV infection were not detected. Results of this study suggest that BVD is not a common cause of mortality in farm-raised white-tailed deer in Pennsylvania.

Key Words: Bovine viral diarrhea virus • Odocoileus virginianus • PCR • persistent infection • white-tailed deer




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T. Passler, P. H. Walz, S. S. Ditchkoff, H. L. Walz, M. D. Givens, and K. V. Brock
Evaluation of hunter-harvested white-tailed deer for evidence of bovine viral diarrhea virus infection in Alabama
J Vet Diagn Invest, January 1, 2008; 20(1): 79 - 82.
[Abstract] [Full Text] [PDF]




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