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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 4, 355-361
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

Development and evaluation of an immunochromatographic strip for detection of Streptococcus Suis type 2 antibody

Junxing Yang, Meilin Jin1, Jianfeng Chen, Ying Yang, Pei Zheng, Anding Zhang, Yunfeng Song, Hongbo Zhou and Huanchun Chen

Correspondence: 1Corresponding Author: Meilin Jin, #1 Shizishan Street, Wuhan, Hubei, P. R. China 430070. jml8328{at}126.com

In this study, an immunochromatographic strip (ICS) was developed for the detection of antibody against Streptococcus suis serotype 2 (SS2). Colloidal gold particles labeled with staphylococcal protein A (SPA), which can bind to the FC fragment of mammalian immunoglobulin, were used as the detector reagent. The capsular polysaccharide (CPS) of SS2 and affinity-purified IgG from a healthy naive pig were immobilized on test and control regions of a nitrocellulose membrane, respectively. The ICS was used to 1) detect anti-CPS antibody in 14 sera taken from 4 SS2-infected pigs, 24 sera from pigs hyperimmunized with SS2, and 68 sera from pigs inoculated or infected with bacteria other than SS2; 2) determine anti-CPS antibody titers of 20 positive sera for comparison with enzyme-linked immunosorbent assay (ELISA); and 3) detect anti-CPS antibody in 226 clinical sera taken from diseased pigs also for comparison with ELISA. An ELISA used as a reference test determined the specificity and sensitivity of the ICS to be 97.1% and 86.3%, respectively. There was excellent agreement between the results obtained by ELISA and the ICS (kappa = 0.843). Additionally, there was strong agreement between the results of bacterial isolation from pig tonsils and ICS test (kappa = 0.658). Because it is rapid and easy to use, the test is suitable for the serological surveillance of SS2 at farms.

Key Words: Antibody detection • capsular polysaccharides • immunochromatographic strip • Streptococcus suis type 2






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