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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 5, 486-491
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

Evaluation of an ovine testis cell line (OA3.Ts) for propagation of capripoxvirus isolates and development of an immunostaining technique for viral plaque visualization

Shawn Babiuk1, Geoff Parkyn, John Copps, June E. Larence, Marta I. Sabara, Timothy R. Bowden, David B. Boyle and R. Paul Kitching

Correspondence: 1Corresponding Author: Shawn Babiuk, National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4, Canada, e-mail: babiuks{at}inspection.gc.ca

An ovine testis cell line (OA3.Ts) was evaluated and compared with primary lamb kidney (LK) cells for its utility in capripoxvirus propagation and titration. A comparison of OA3.Ts cell growth kinetics and morphology at low (<33) and high (34–36) passage levels indicated a difference in both characteristics. However, viral titers determined in low and high passage OA3.Ts cells were comparable with those obtained using LK cells. Capripoxvirus infection of OA3.Ts and LK cells resulted in a similar cytopathic effect, which allowed for the detection of discrete viral plaques following immunostaining with capripoxvirus-specific antiserum.

Key Words: Capripoxvirus • immunostaining • ovine testis cells (OA3.Ts)




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S. Babiuk, T. R. Bowden, G. Parkyn, B. Dalman, D. M. Hoa, N. T. Long, P. P. Vu, D. X. Bieu, J. Copps, and D. B. Boyle
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