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Journal of Veterinary Diagnostic Investigation Vol. 19 Issue 5, 539-544
Copyright © 2007 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communications

Bovine serum panel for evaluating foot-and-mouth disease virus nonstructural protein antibody tests

Satya Parida, Lucy Fleming, Debi Gibson, Pip A. Hamblin, Santina Grazioli, Emiliana Brocchi and David J. Paton1

Correspondence: 1Corresponding Author: David J. Paton, Institute for Animal Health, Pirbright Laboratory, Ash Road, Woking, Surrey GU24 0NF, UK, e-mail: david.paton{at}bbsrc.ac.uk

A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination. Virus replication and persistence had been characterized in all of the animals. The proportion of the sera scored positive by 5 tests for antibodies to the nonstructural proteins of FMDV varied, suggesting that the panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of this panel will help in assessment of new tests and quality control of existing methods.

Key Words: Bovine serum panel • foot-and-mouth disease virus • nonstructural protein antibody assays




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J. Perkins, S. Parida, and A. Clavijo
Use of a Standardized Bovine Serum Panel To Evaluate a Multiplexed Nonstructural Protein Antibody Assay for Serological Surveillance of Foot-and-Mouth Disease
Clin. Vaccine Immunol., November 1, 2007; 14(11): 1472 - 1482.
[Abstract] [Full Text] [PDF]


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