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Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 1, 58-60
Copyright © 2008 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Communications

Comparison of retropharyngeal lymph node and obex region of the brainstem in detection of chronic wasting disease in white-tailed deer (Odocoileus virginianus)

Delwyn P. Keane1, Daniel J. Barr, Jason E. Keller, S. Mark Hall, Julie A. Langenberg and Philip N. Bochsler

Correspondence: 1Corresponding Author: Wisconsin Veterinary Diagnostic Laboratory, University of Wisconsin, 445 Easterday Lane, Madison, WI 53706, e-mail: Delwyn.Keane{at}wvdl.wisc.edu

Chronic wasting disease (CWD) in Wisconsin was first identified in February 2002. By April 2005, medial retropharyngeal lymph node (RLN) tissues had been examined from over 75,000 white-tailed deer for the presence of CWD by either immunohistochemical (IHC) staining for the prion protein associated with CWD (PrPres) or by using enzyme-linked immunosorbent assays with confirmation of positives by IHC staining and had been detected in 469 animals. Obex tissue was also available from 438 of the CWD-positive animals and was CWD positive by IHC staining in 355 (81%). To verify whether false-negative results were possible examining only RLN, both obex and RLN samples were examined for CWD by IHC staining from 4,430 of the white-tailed deer harvested from an area in Wisconsin where the overall deer CWD prevalence was approximately 6.2%. Two hundred and fourteen of the 269 positive deer (79.6%) had deposits of PrPres in both obex and lymphoid tissues, 55 (20.4%) had deposits only in lymphoid tissue, and there were no deer that had deposits only in obex.

Key Words: Chronic wasting disease • enzyme-linked immunosorbent assay • immunohistochemistry • prion • white-tailed deer




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