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Culture of Piscirickettsia salmonis on enriched blood agar

Correspondence: ¹Corresponding Author: Michael J. Mauel, Thad Cochran National Warmwater Aquaculture Center, College of Veterinary Medicine, Mississippi State University, PO Box 197, Stoneville, MS 38776, e-mail: mauel{at}cvm.msstate.edu

Piscirickettsia salmonis is the etiologic agent of piscirickettsiosis, an economically significant disease of fish. Isolation of P. salmonis by culturing on fish cell lines has been the standard technique since the initial isolation of the organism. The ability to grow P. salmonis on artificial media would relieve facilities of the cost of maintaining cell lines, permit isolation at fish culture sites with fewer contamination problems, and allow easier transport of isolates to diagnostic facilities for confirmation assays. This report describes the successful culture of P. salmonis on enriched blood agar.

Key Words: Culture • enriched blood agar • Piscirickettsia