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Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 4, 393-413
Copyright © 2008 by the American Association of Veterinary Laboratory Diagnosticians
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Review Articles

Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories

José A. Ramos-Vara1, Matti Kiupel2, Timothy Baszler, Laura Bliven, Bruce Brodersen, Brian Chelack, Stefanie Czub, Fabio Del Piero, Sharon Dial, E.J. Ehrhart, Tanya Graham, Lisa Manning, Daniel Paulsen, Victor E. Valli and Keith West

Correspondence: 1Corresponding Author: José A. Ramos-Vara, Animal Disease Diagnostic Laboratory, Purdue University, 406 South University, West Lafayette, IN 47907. ramosja{at}purdue.edu

Correspondence: 2Dr. Kiupel is Chair of the AAVLD Subcommittee on Standardization of IHC.

This document is the consensus of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) Subcommittee on Standardization of Immunohistochemistry on a set of guidelines for immunohistochemistry (IHC) testing in veterinary laboratories. Immunohistochemistry is a powerful ancillary methodology frequently used in many veterinary laboratories for both diagnostic and research purposes. However, neither standardization nor validation of IHC tests has been completely achieved in veterinary medicine. This document addresses both issues. Topics covered include antibody selection, fixation, antigen retrieval, antibody incubation, antibody dilutions, tissue and reagent controls, buffers, and detection systems. The validation of an IHC test is addressed for both infectious diseases and neoplastic processes. In addition, storage and handling of IHC reagents, interpretation, quality control and assurance, and troubleshooting are also discussed. Proper standardization and validation of IHC will improve the quality of diagnostics in veterinary laboratories.

Key Words: Animal diseases • diagnostic immunohistochemistry • standardization • validation






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