HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Leuwerke, B. Articles by Thacker, E. Search for Related Content PubMed PubMed Citation Articles by Leuwerke, B. Articles by Thacker, E.

Comparison of three serological assays to determine the cross-reactivity of antibodies from eight genetically diverse U.S. Swine influenza viruses

Correspondence: ¹Corresponding Author: Eileen Thacker, NPL, Animal Health, USDA-ARS, 5601 Sunnyside Avenue, Beltsville, MD 20705-5148. eileen.thacker{at}ars.usda.gov

Swine influenza virus is an economically important pathogen to the U.S. swine industry. New influenza subtypes and isolates within subtypes with different genetic and antigenic makeup have recently emerged in U.S. swineherds. As a result of the emergence of these new viruses, diagnosticians' ability to accurately diagnose influenza infection in pigs and develop appropriate vaccine strategies has become increasingly difficult. The current study compares the ability of subtype-specific commercial enzyme-linked immunosorbent assays (ELISA), hemagglutination inhibition (HI), and serum neutralization (SN) assays to detect antibodies elicited by multiple isolates within different subtypes of influenza virus. Pigs were infected with genetically and antigenically different isolates of the 3 major circulating subtypes within populations of swine (H1N1, H1N2, and H3N2). Serum was collected when all pigs within a group collectively reached HI reciprocal titers 160 against that group's homologous challenge virus. The antibody cross-reactivity of the sera between isolates was determined using ELISA, HI, and SN assays. In addition, the correlation between the 3 assays was determined. The assays differed in their ability to detect antibodies produced by the viruses used in the study. The results provide important information to diagnostic laboratories, veterinarians, and swine producers on the ability of 3 common serological assays used in identifying infection with influenza in pigs.

Key Words: Enzyme-linked immunosorbent assay • hemagglutination inhibition assay • pigs • serum neutralizing assay • Swine influenza viruses

This article has been cited by other articles:

				M. Torremorell, A. Juarez, E. Chavez, J. Yescas, J. M. Doporto, and M. Gramer Procedures to eliminate H3N2 swine influenza virus from a pig herd Vet Rec., July 18, 2009; 165(3): 74 - 77. [Abstract] [Full Text] [PDF]