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Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 5, 593-597
Copyright © 2008 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

Evaluation of three methods for measurement of hemoglobin and calculated hemoglobin variables with the ADVIA 120® and ADVIA 2120® systems in goats

Natali Bauer1 and Andreas Moritz

Correspondence: 1Corresponding Author: Natali Bauer, Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig University Giessen, Frankfurterstr. 129, 35392 Giessen, Germany. Natalie.Bauer{at}vetmed.uni-giessen.de

The present study investigated 3 methods of hemoglobin (Hb) determination in goats using the ADVIA 120 and ADVIA 2120 systems. Ethylenediaminetetraacetic acid anticoagulated caprine blood samples (n = 40 goats) were subjected to Hb determination via the cyanmethemoglobin methods in both instruments and a novel, cyanide-free, colorimetric method with the ADVIA 2120. Statistical analysis of the data included a linear regression, Passing-Bablok regression, and Bland-Altman diagram. Colorimetric Hb results determined with both analyzers had excellent correlation (r = 0.98); however, a mean proportional bias of –19.1% was present in comparison to the reference method. There also was excellent agreement between cellular Hb concentrations when measured with both analyzers (r = 0.96), and the constant bias was close to zero. However, imprecision was higher compared to colorimetric methods. Excellent to fair agreement was evident for all calculated erythrocyte and Hb variables. Because of the excellent correlation between the ADVIA 120 and ADVIA 2120, the cyanide-free method of Hb determination could be used with caprine blood specimens; however, the proportional bias must be considered.

Key Words: ADVIA 120 • ADVIA 2120 • caprine • correlation • cyanide-free • flow cytometry • hematology • hemoglobin • method validation






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