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Journal of Veterinary Diagnostic Investigation Vol. 20 Issue 5, 598-605
Copyright © 2008 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease

Recai Tunca1, Mahmut Sozmen, Hidayet Erdogan, Mehmet Citil, Erdogan Uzlu, Hasan Ozen and Erhan Gokçe

Correspondence: 1Corresponding Author: Recai Tunca, Department of Pathology, Faculty of Veterinary Medicine, University of Adnan Menderes, 0916 Isikli, Aydin, Turkey. rtunca26{at}hotmail.com

The current study was designed to determine the changes of the cardiac troponin I (cTnI) expression in blood and tissue during the myocardial degeneration in calves with foot-and-mouth disease (FMD). Seventeen crossbred calves presenting pathological signs for FMD confirmed by viral analysis were studied. A biochemistry panel and immunohistochemistry were performed on 17 diseased calves and 7 calves used as controls. Creatine kinase (CK), CK-myocardial band (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities were analyzed for both groups. Cardiac troponin I levels were measured by a commercially available enzyme-linked immunosorbent assay kit. Mean cTnI (14.8 ± 1.9 ng/ml) concentration and CK (573 ± 407 U/l), CK-MB (238 ± 37 U/l), AST (84 ± 7), and LDH (298 ± 29 U/l) activities were higher in FMD cases compared with controls. Immunohistochemistry revealed loss or depletion of cTnI expression in myocardium of all cases. None of the 7 controls showed loss of cTnI expression. Increased serum cTnI concentration correlated with myocardial injury and loss of cTnI immunolabeling in cardiomyocytes of calves with FMD.

Key Words: Aspartate aminotransferase • Calves • cardiac troponin I • creatine kinase • foot-and-mouth disease • immunohistochemistry • lactate dehydrogenase • myocardial injury • serology






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