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Evaluation of a SRS2 sandwich commercial enzyme-linked immunosorbent assay for the detection of anti–Neospora caninum antibodies in bovine and canine sera

Correspondence: ¹Corresponding Author: Bertrand Losson, University of Liege, Faculty of Veterinary Medicine, Laboratory of Parasitology, Sart Tilman B43, B-4000 Liege, Belgium. blosson{at}ulg.ac.be

Neospora caninum is a parasite responsible for abortion in cows and neuromuscular disease in dogs. Serology is the most widely used technique to evaluate the prevalence of N. caninum in different host populations. A sandwich enzyme-linked immunosorbent assay (ELISA) based on the use of an anti-SRS2 monoclonal antibody was evaluated against the indirect fluorescent antibody test for 100 canine sera and against a well-characterized ELISA for 102 bovine sera. In cattle sera, the relative sensitivity and relative specificity were 100%. In dog sera, the relative specificity and relative sensitivity were 94% and 86%, respectively. The kappa value was 1 for bovine sera and 0.77 for canine sera. The seroprevalence was 3.9% in bovine sera and 21–23% in canine sera. The SRS2 sandwich ELISA was considered a valuable tool in both species.

Key Words: Cattle • dogs • enzyme-linked immunosorbent assay • Neospora caninum • serology • SRS2 monoclonal antibody