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Journal of Veterinary Diagnostic Investigation Vol. 21 Issue 4, 499-503
Copyright © 2009 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Research Reports

Neutralizing monoclonal antibody sandwich liquid-phase blocking enzyme-linked immunosorbent assay for detection of Foot-and-mouth disease virus type O antibodies

Kazuki Morioka1, Katsuhiko Fukai, Kazuo Yoshida, Reiko Yamazoe, Hiroyuki Onozato, Seiichi Ohashi, Tomoyuki Tsuda and Kenichi Sakamoto

Correspondence: 1Corresponding Author: Kazuki Morioka, Exotic Diseases Research Station, National Institute of Animal Health, Josuihoncho 6-20-1 Kodaira Tokyo 187-0022, Japan. morioka{at}affrc.go.jp

Liquid-phase blocking enzyme-linked immunosorbent assay (LPBE) using the neutralizing monoclonal antibody (mAb) sandwich method (M-LPBE) for detection of Foot-and-mouth disease virus (FMDV) type O antibodies was developed. Two neutralizing mAbs, 72C1 and 65H6, were raised against the FMDV O/JPN/2000 strain, and used as trapping and peroxidase-labeled detecting antibodies, respectively. Sera from animals experimentally infected with FMDV showed specific positive results by M-LPBE, which were correlated with the results of the virus neutralization test (VNT). When 303 negative bovine and 302 negative swine sera were tested, the specificity of M-LPBE was 100% and 99.7%, respectively. In addition, nine samples that had been collected in 2000 in Japan and regarded as evidently false positives by LPBE (supplied by the World Reference Laboratory for Foot-and-Mouth Disease) were uniformly negative by M-LPBE, just like VNT. Therefore, M-LPBE seems to have sufficient specificity for FMDV type O antibody screening and diagnosis.

Key Words: Enzyme-linked immunosorbent assay • Foot-and-mouth disease virus • monoclonal antibody






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