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Journal of Veterinary Diagnostic Investigation Vol. 21 Issue 4, 510-516
Copyright © 2009 by the American Association of Veterinary Laboratory Diagnosticians
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Brief Research Reports

Genotypes, antibiogram, and pulsed-field gel electrophoresis profiles of Escherichia coli strains from piglets in Korea

Su In Lee, Nabin Rayamahji, Won Jung Lee, Seung Bin Cha, Min Kyung Shin, Yu Mi Roh and Han Sang Yoo1

Correspondence: 1Corresponding Author: Han Sang Yoo, Department of Infectious Disease, College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University, Seoul 152-742, Korea, e-mail: yoohs{at}snu.ac.kr

Adherence factors and enterotoxins are major virulence factors of enterotoxigenic Escherichia coli (ETEC). Antibiotics have been used frequently for the treatment and prevention of ETEC infection in piggeries worldwide, including Korea. Therefore, data on both virulence profiles and antibiotic resistance patterns are useful in the epidemiological study of ETEC. A total number of 198 E. coli field isolates were examined. The most prevalent pathotype was F1, followed by a combination of F1 and EAST1. All of the 71 isolates were resistant to more than 2 antibiotics used in a disk diffusion test, and 87.94% of the isolates were found to be resistant to more than 4 antibiotics. Investigations were also conducted to correlate the virulence gene profiles with antibiogram and pulsed-field gel electrophoresis (PFGE). Although a high degree of polymorphism was noted among strains having the same virulence patterns, the highest similarity pattern was observed carrying the same virulence profiles and similar antibiogram. Thus, investigation of both virulence profiles and antibiogram is essential to the epidemiological study of ETEC. Moreover, the PFGE method might be applicable as a tool to reveal genetic relatedness among E. coli strains from piggeries in Korea.

Key Words: Antibiogram • Escherichia coli • genotype • pulsed-field gel electrophoresis






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