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Assessment of prothrombin time, activated partial thromboplastin time, and fibrinogen concentration on equine plasma samples following different storage conditions

Correspondence: ¹Corresponding Author: Giuseppe Piccione, Dipartimento di Scienze Sperimentali e Biotecnologie Applicate, Sezione di Fisiologia Applicata ed Etologia Comparata, Facoltà di Medicina Veterinaria, Polo Universitario dell'Annunziata, Università degli Studi di Messina, 98168 Messina, Italy. giuseppe.piccione{at}unime.it

The aim of the present study was to assess the effect of different storage conditions on prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen concentration in clinical samples from healthy horses. A total of 100 healthy horses of varying breeds and gender, ranging in age from 4 to 18 years, with a mean body weight of 480 ± 70 kg, were used. Blood was collected by jugular venipuncture, and a hemochrome-cytometric examination was conducted on all samples. All blood samples were centrifuged and divided into 4 different aliquots to assess clotting parameters by means of a coagulometer. The first aliquots were analyzed 1 hr after collection, the second aliquots were refrigerated at 8°C for 6 hr, the third aliquots were frozen at –20°C for 24 hr, and the fourth aliquots were frozen at –20°C for 48 hr. Significant differences (P < 0.05) were determined by one-way analysis of variance with repeated measures, and statistical analysis showed a significant effect of the experimental conditions on all parameters studied. In particular, the results demonstrated that coagulation tests can be done within 6 hr when samples are stored at 8°C because the short-term refrigeration does not change the result of analyses; storage at –20°C is acceptable only after 24 hr for PT, aPTT, and fibrinogen measurements because after 48 hr, freezing alters the values of clotting parameters. Therefore, the results of this investigation indicate that clotting parameters remain stable only up to 24 hr in horses without adversely affecting hemostasis test results.

Key Words: Activated partial thromboplastin time • fibrinogen • horses • prothrombin time • storage conditions