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Reference intervals and method optimization for variables reflecting hypocoagulatory and hypercoagulatory states in dogs using the STA Compact® automated analyzer

Correspondence: ¹Corresponding Author: Natali Bauer, Department of Veterinary Clinical Sciences, Clinical Pathophysiology and Clinical Pathology, Justus-Liebig University Giessen, Frankfurterstr. 126, 35392 Giessen, Germany. Natali.Bauer{at}vetmed.uni-giessen.de

Reference intervals for coagulation parameters have been rarely determined in dogs for the STA Compact® automated coagulation analyzer, so it is the aim of the current study to validate assays and establish reference ranges for its use in canine specimens. Coagulation parameters were assessed in 56 healthy dogs with a median age of 2 years and evenly distributed sex. The 95% reference intervals were as follows: 1-stage prothrombin time = 5.7–8.0 sec; activated partial thromboplastin time (APTT) = 10.0–14.3 sec; thrombin time (TT) = 11.9–18.3 sec; fibrinogen = 1.3–3.1 g/l; antithrombin (AT) = 107.9–128.0%; D-dimer = 0.023–0.65 µg/ml; anti-factor Xa = 0.04–0.26 IU/l; and activated protein C (APC) ratio = 2.0–3.0. Protein C and S activity was markedly below (<–20%) and factor VIII was 2- to 11-fold above the human calibration standard, so a standard curve had to be prepared from canine pooled plasma. Reference intervals for protein C, protein S, and factor VIII were 75.5–118.9%, 74.4–160.5%, and 70.9–136.4%, respectively, compared with a canine standard curve. Streptokinase-activated plasminogen assay was not suitable for dogs. There was no significant impact of sex on hemostasis test results. Factor VIII activity, AT, protein C, protein S, and APC ratio were overestimated in hemolytic plasma, whereas fibrinogen, TT, and APTT were underestimated. Lipemia resulted only in false-high D-dimers. This study provided useful reference intervals for dogs, but some human tests (i.e., protein C, protein S, factor VIII, and plasminogen) required modification.

Key Words: Dogs • fibrinolysis • hemostasis • interferences • sex • thrombophilia