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Journal of Veterinary Diagnostic Investigation Vol. 22 Issue 1, 10-19
Copyright © 2010 by the American Association of Veterinary Laboratory Diagnosticians
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Full Scientific Reports

Development and application of an indirect immunoperoxidase assay for the detection of Duck swollen head hemorrhagic disease virus antigen in Pekin ducks (Anas platyrhynchos)

Chuanfeng Li, Chanjuan Shen, Anchun Cheng1, Mingshu Wang, Na Zhang, Yi Zhou, Dekang Zhu, Renyong Jia, Qihui Luo and Xiaoyue Chen

Correspondence: 1Corresponding Author: Anchun Cheng, College of Veterinary Medicine of Sichuan Agricultural University, Avian Disease Research Center, Yaan, Sichuan Province, China 625014, e-mail: chenganchun{at}vip.163.com, e-mail: lichuanfeng{at}126.com

An improved indirect immunoperoxidase assay (IPA) was developed to detect antigens of Duck swollen head hemorrhagic disease virus (DSHDV) in paraformaldehyde-fixed, paraffin-embedded tissues of Pekin ducks (Anas platyrhynchos). This technique used an indirect streptavidin–alkaline phosphatase labeling system with polyclonal antiserum developed against purified DSHDV antigens. Specimens from the experimentally inoculated Pekin ducks with DSHDV and archived paraffin-embedded tissues from natural cases of Duck viral swollen head hemorrhagic disease (DVSHD) were examined by clinical and histological criteria. Positive staining was most widely observed in the cytoplasm of the following organs: immune, digestive, and urinary organs, heart, lung, and trachea, which corresponded to the intracellular distribution of reovirus. The DSHDV antigens were first detected at 4 hr postinoculation in the bursa of Fabricius of infected ducks. Therefore, this method was suitable for the early diagnosis of DVSHD. Immunoperoxidase staining was not present in tissues and organs of sham-inoculated ducks (negative control). The IPA developed in the current study is a convenient, sensitive, and specific means of detecting DSHDV and is applicable to routine diagnosis, retrospective studies, and prospective studies of DSHDV infection in ducks.

Key Words: Duck swollen head hemorrhagic disease virus • immunoperoxidase assay • reovirus






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