JVDI Advertisement
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ellis, J.
Right arrow Articles by Haines, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ellis, J.
Right arrow Articles by Haines, D.
Journal of Veterinary Diagnostic Investigation, Vol 7, Issue 4, 433-436
Copyright © 1995 by American Association of Veterinary Laboratory Diagnosticians

Articles

Comparison of detection methods for bovine viral diarrhea virus in bovine abortions and neonatal death

JA Ellis, K Martin, GR Norman, and DM Haines

Diagnostic Virology Laboratory, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.

The objective of this study was to examine a monoclonal antibody-based immunohistochemical staining method for its efficacy in diagnosis of bovine virus diarrhea virus (BVDV)-induced abortion and neonatal calf death. This method was compared to viral isolation and immunofluorescence staining of frozen tissue sections. Tissues from 105 cases, 53 fetuses and 52 neonates, were tested by the 3 methods. There were significant numbers of both false negatives and false positives with the immunofluorescence method and significant numbers of false negatives with the viral isolation method. Of the methods tested, immunohistochemical staining using monoclonal antibody 15C5 performed best, differentiating 97% of positive and negative cases. These results indicated that immunohistochemical staining can be applied to improve the accuracy of BVDV diagnosis in cases of abortion and perinatal death.


This article has been cited by other articles:


Home page
 jvdiHome page
A. L. Garcia-Perez, E. Minguijon, J. F. Barandika, G. Aduriz, I. Povedano, R. A. Juste, and A. Hurtado
Detection of Border disease virus in fetuses, stillbirths, and newborn lambs from natural and experimental infections
J Vet Diagn Invest, May 1, 2009; 21(3): 331 - 337.
[Abstract] [Full Text] [PDF]

Home page
 jvdiHome page
J. F. Ridpath, Y.-W. Chiang, J. Waldbillig, and J. D. Neill
Stability of Bovine viral diarrhea virus antigen in ear punch samples collected from bovine fetuses
J Vet Diagn Invest, May 1, 2009; 21(3): 346 - 349.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
P. Aich, H. L. Wilson, R. S. Kaushik, A. A. Potter, L. A. Babiuk, and P. Griebel
Comparative analysis of innate immune responses following infection of newborn calves with bovine rotavirus and bovine coronavirus
J. Gen. Virol., October 1, 2007; 88(10): 2749 - 2761.
[Abstract] [Full Text] [PDF]

Home page
 jvdiHome page
M. Hilbe, H. Stalder, E. Peterhans, M. Haessig, M. Nussbaumer, C. Egli, C. Schelp, K. Zlinszky, and F. Ehrensperger
Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves
J Vet Diagn Invest, January 1, 2007; 19(1): 28 - 34.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the American Association of Veterinary Laboratory Diagnosticians, Inc.