| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
National Animal Disease Center, USDA-ARS, Ames, IA 50010, USA.
Peripheral blood mononuclear cells were isolated from noninfected control cows and from cows with either subclinical or clinical paratuberculosis (Johne's disease). Cells were incubated for 6, 12, 24, and 48 hours in complete medium with the following mitogens: concanavalin A (ConA), phytohemagglutinin-P (PHAP), pokeweed mitogen (PWM), and Escherichia coli lipopolysaccharide. In addition, cells were incubated for the same time periods with a Mycobacterium paratuberculosis sonicate (MpS) and live and heat-killed M. paratu-berculosis at 10:1 bacteria: cell ratio. After incubation, cell-free supernatants were analyzed for gamma-interferon (gamma-IFN) production. Cells from subclinical cows produced significantly higher levels of gamma-IFN than did cells from clinical animals after stimulation with mitogens ConA, PHAP, and PWM. Levels of gamma-IFN produced by noninfected control animals generally followed the pattern of those of subclinical animals. After incubation with MpS, significantly greater quantities of gamma-IFN were produced by cells isolated from subclinical animals than by cells from clinical cows and noninfected controls. Stimulation of cells with heat-killed or live M. paratuberculosis evoked a similar response. This study indicates that gamma-IFN production by peripheral blood mononuclear cells in response to M. paratuberculosis antigen may be an important diagnostic tool for the detection of paratuberculosis in subclinically affected animals.
This article has been cited by other articles:
|
|
 |
|
  K. R. Lybeck, A. K. Storset, and I. Olsen Neutralization of Interleukin-10 from CD14+ Monocytes Enhances Gamma Interferon Production in Peripheral Blood Mononuclear Cells from Mycobacterium avium subsp. paratuberculosis-Infected Goats Clin. Vaccine Immunol., July 1, 2009; 16(7): 1003 - 1011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hinger, H. Brandt, S. Horner, and G. Erhardt Short Communication: Association Analysis of Microsatellites and Mycobacterium avium Subspecies paratuberculosis Antibody Response in German Holsteins J Dairy Sci, April 1, 2007; 90(4): 1957 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Stabel, K. Kimura, and S. Robbe-Austerman Augmentation of secreted and intracellular gamma interferon following johnin purified protein derivative sensitization of cows naturally infected with Mycobacterium avium subsp. paratuberculosis J Vet Diagn Invest, January 1, 2007; 19(1): 43 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Semret, D. Bakker, N. Smart, I. Olsen, K. Haslov, and M. A. Behr Genetic Analysis of Mycobacterium avium Complex Strains Used for Producing Purified Protein Derivatives Clin. Vaccine Immunol., September 1, 2006; 13(9): 991 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Stabel and J. P. Bannantine Development of a Nested PCR Method Targeting a Unique Multicopy Element, ISMap02, for Detection of Mycobacterium avium subsp. paratuberculosis in Fecal Samples J. Clin. Microbiol., September 1, 2005; 43(9): 4744 - 4750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tanaka, M. Sato, T. Onitsuka, H. Kamata, and Y. Yokomizo Inflammatory Cytokine Gene Expression in Different Types of Granulomatous Lesions during Asymptomatic Stages of Bovine Paratuberculosis Veterinary Pathology, September 1, 2005; 42(5): 579 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Shin, C.-F. Chang, C.-D. Chang, S. P. McDonough, B. Thompson, H. S. Yoo, and Y.-F. Chang In Vitro Cellular Immune Responses to Recombinant Antigens of Mycobacterium avium subsp. paratuberculosis Infect. Immun., August 1, 2005; 73(8): 5074 - 5085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Coussens Model for Immune Responses to Mycobacterium avium Subspecies paratuberculosis in Cattle Infect. Immun., June 1, 2004; 72(6): 3089 - 3096. [Full Text] [PDF]
|
|
|
|
|
|
M. S. Khalifeh and J. R. Stabel Effects of Gamma Interferon, Interleukin-10, and Transforming Growth Factor {beta} on the Survival of Mycobacterium avium subsp. paratuberculosis in Monocyte-Derived Macrophages from Naturally Infected Cattle Infect. Immun., April 1, 2004; 72(4): 1974 - 1982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Buza, H. Hikono, Y. Mori, R. Nagata, S. Hirayama, A. M. Bari, Y. Shu, N. M. Tsuji, and E. Momotani Neutralization of Interleukin-10 Significantly Enhances Gamma Interferon Expression in Peripheral Blood by Stimulation with Johnin Purified Protein Derivative and by Infection with Mycobacterium avium subsp. paratuberculosis in Experimentally Infected Cattle with Paratuberculosis Infect. Immun., April 1, 2004; 72(4): 2425 - 2428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Buza, Y. Mori, A. M. Bari, H. Hikono Aodon-geril, S. Hirayama, Y. Shu, and E. Momotani Mycobacterium avium subsp. paratuberculosis Infection Causes Suppression of RANTES, Monocyte Chemoattractant Protein 1, and Tumor Necrosis Factor Alpha Expression in Peripheral Blood of Experimentally Infected Cattle Infect. Immun., December 1, 2003; 71(12): 7223 - 7227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Waters, J. M. Miller, M. V. Palmer, J. R. Stabel, D. E. Jones, K. A. Koistinen, E. M. Steadham, M. J. Hamilton, W. C. Davis, and J. P. Bannantine Early Induction of Humoral and Cellular Immune Responses during Experimental Mycobacterium avium subsp. paratuberculosis Infection of Calves Infect. Immun., September 1, 2003; 71(9): 5130 - 5138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Weiss, O. A. Evanson, A. Moritz, M. Q. Deng, and M. S. Abrahamsen Differential Responses of Bovine Macrophages to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium Infect. Immun., October 1, 2002; 70(10): 5556 - 5561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koets, V. Rutten, A. Hoek, F. van Mil, K. Muller, D. Bakker, E. Gruys, and W. van Eden Progressive Bovine Paratuberculosis Is Associated with Local Loss of CD4+ T Cells, Increased Frequency of {gamma}{delta} T Cells, and Related Changes in T-Cell Function Infect. Immun., July 1, 2002; 70(7): 3856 - 3864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Jungersen, A. Huda, J. J. Hansen, and P. Lind Interpretation of the Gamma Interferon Test for Diagnosis of Subclinical Paratuberculosis in Cattle Clin. Vaccine Immunol., March 1, 2002; 9(2): 453 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Olsen, M. Tryland, H. G. Wiker, and L. J. Reitan AhpC, AhpD, and a Secreted 14-Kilodalton Antigen from Mycobacterium avium subsp. paratuberculosis Distinguish between Paratuberculosis and Bovine Tuberculosis in an Enzyme-Linked Immunosorbent Assay Clin. Vaccine Immunol., July 1, 2001; 8(4): 797 - 801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. B. Harris and R. G. Barletta Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine Clin. Microbiol. Rev., July 1, 2001; 14(3): 489 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Weiss, O. A. Evanson, D. J. McClenahan, M. S. Abrahamsen, and B. K. Walcheck Regulation of Expression of Major Histocompatibility Antigens by Bovine Macrophages Infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium Infect. Immun., February 1, 2001; 69(2): 1002 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Olsen, L. J. Reitan, G. Holstad, and H. G. Wiker Alkyl Hydroperoxide Reductases C and D Are Major Antigens Constitutively Expressed by Mycobacterium avium subsp. paratuberculosis Infect. Immun., February 1, 2000; 68(2): 801 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Waters, J. R. Stabel, R. E. Sacco, J. A. Harp, B. A. Pesch, and M. J. Wannemuehler Antigen-Specific B-Cell Unresponsiveness Induced by Chronic Mycobacterium avium subsp. paratuberculosis Infection of Cattle Infect. Immun., April 1, 1999; 67(4): 1593 - 1598. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
